Sandra Johnen

Department of Ophthalmology

University Hospital RWTH Aachen

BIOGRAPHY

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PUBLICATIONS

Antiangiogenic and Neurogenic Activities of Sleeping Beauty-Mediated PEDF-Transfected RPE Cells In Vitro and In Vivo.

BioMed research international , 2015 | Pubmed ID: 26697494

Properties of Retinal Precursor Cells Grown on Vertically Aligned Multiwalled Carbon Nanotubes Generated for the Modification of Retinal Implant-Embedded Microelectrode Arrays.

Journal of ophthalmology , 2016 | Pubmed ID: 27200182

Engineering of PEDF-Expressing Primary Pigment Epithelial Cells by the SB Transposon System Delivered by pFAR4 Plasmids.

Molecular therapy. Nucleic acids Mar, 2017 | Pubmed ID: 28325297

Going non-viral: the Sleeping Beauty transposon system breaks on through to the clinical side.

Critical reviews in biochemistry and molecular biology 08, 2017 | Pubmed ID: 28402189

Long-Term PEDF Release in Rat Iris and Retinal Epithelial Cells after Sleeping Beauty Transposon-Mediated Gene Delivery.

Molecular therapy. Nucleic acids , | Pubmed ID: 29246287

Cryopreservation of amniotic membrane with and without glycerol additive.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie Jun, 2018 | Pubmed ID: 29623460

The Antibiotic-free pFAR4 Vector Paired with the Sleeping Beauty Transposon System Mediates Efficient Transgene Delivery in Human Cells.

Molecular therapy. Nucleic acids , | Pubmed ID: 29858090

The very large electrode array for retinal stimulation (VLARS)-A concept study.

Journal of neural engineering 11, 2019 | Pubmed ID: 31480027

Preclinical Evaluation of a Cell-Based Gene Therapy Using the Sleeping Beauty Transposon System in Choroidal Neovascularization.

Molecular therapy. Methods & clinical development , | Pubmed ID: 31890733

Establishment and Characterization of a Unilateral UV-Induced Photoreceptor Degeneration Model in the C57Bl/6J Mouse.

Translational vision science & technology 08, 2020 | Pubmed ID: 32879777

INSTITUTIONS

University Hospital RWTH Aachen

JOVE ARTICLES

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Sandra Johnen¹,

Nina Harmening^2,3,

Corinne Marie^4,5,

Daniel Scherman⁴,

Zsuzsanna Izsvák⁶,

Zoltán Ivics⁷,

Peter Walter¹,

Gabriele Thumann^2,3

¹Department of Ophthalmology, University Hospital RWTH Aachen,

²Experimental Ophthalmology, University of Geneva,

³Department of Ophthalmology, University Hospitals of Geneva,

⁴, Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé,

⁵Chimie ParisTech, PSL Research University,

⁶, Max Delbrück Center for Molecular Medicine in the Helmholtz Association,

⁷Division of Medical Biotechnology, Paul-Ehrlich-Institute

Medicine

Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy

Thais Bascuas^*,1,2,

Martina Kropp^*,1,2,

Nina Harmening^1,2,

Brittany M. Wong¹,

Sandra Johnen³,

Zsuzsanna Izsvák⁴,

Gabriele Thumann^1,2

¹Experimental Ophthalmology, University of Geneva,

²Department of Ophthalmology, University Hospitals of Geneva,

³Department of Ophthalmology, University Hospital RWTH Aachen,

⁴, Max Delbrück Center for Molecular Medicine

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