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A Heterotopic Mouse Model for Studying Laryngeal Transplantation

Laryngeal heterotopic transplantation, although a technically challenging procedure, offers more scientific analysis and cost benefits compared to other ...

Mayo Clinic Arizona

The deterioration of immune function with advancing age is associated with an increased incidence of cancer. Most of the studies to evaluate the effect of immunotherapy on cancer have been conducted in the young without considering the effect of age-associated changes in immune function. Studies from my laboratory and others groups indicate that immunotherapeutic interventions could be effective in young animals, but that the same therapies are not as effective in old animals. The present review summarizes some defects found in the old immune system affecting the activation of antitumor immune responses, the strategies used to activate a more robust antitumor immune response in the old and the description of a preclinical tumor model indicating possible strategies for optimization of immunotherapeutic interventions in the old.

Immunity, cancer and aging: lessons from mouse models.

The neurotrophic potential of human platelet lysate substitution for fetal bovine serum in glial induction culture medium.

To gain insight into the molecular mechanisms underlying the early stages of vocal fold extracellular matrix (ECM) remodeling after a mid-membranous injury resulting from the use of human amniotic epithelial cells (hAEC), as a novel regenerative medicine cell-based therapy.

Human amniotic epithelial cell transplantation improves scar remodeling in a rabbit model of acute vocal fold injury: a pilot study.

Develop a patient-specific tissue engineered construct for laryngeal reconstruction following a partial laryngectomy.

A Tissue Engineered Construct for Laryngeal Regeneration: A Proof-of-Concept Device Design Study.

The lamina propria within the vocal fold (VF) is a complex multilayered tissue that increases in stiffness from the superficial to deep layer, where this characteristic is crucial for VF sound production. Tissue-engineered scaffolds designed for VF repair must mimic the biophysical nature of the native vocal fold and promote cell viability, cell spreading, and vibration with air flow. In this study, we present a unique trilayered, partially degradable hydrogel scaffold that mimics the multilayered structure of the VF lamina propria. Using thiol-norbornene photochemistry, trilayered hydrogel scaffolds were fabricated via layer-by-layer stacking with increasing polymer concentration from the top to middle to deep layer. Mechanical analysis confirmed that hydrogel modulus increased with increasing polymer concentration. Partially degradable hydrogels promoted high cell viability and cell spreading in three dimensions as assessed via live/dead and cytoskeleton staining, respectively. Importantly, partially degradable hydrogels maintained some degree of the three dimensional polymer network following protease exposure, while still enabling encapsulated cells to remodel their local environment via protease secretion. Finally, the trilayered hydrogel scaffold successfully vibrated and produced sound in proof-of-concept air flow studies. This work represents a critical first step toward the design of a multilayered, hydrogel scaffold for vocal fold tissue engineering.

Cheryl Myers

Head and Neck Regenerative Medicine Laboratory,
Center for Regenerative Medicine,
Head and Neck Regenerative Medicine Laboratory, Center for Regenerative Medicine

Mayo Clinic Arizona

A Heterotopic Mouse Model for Studying Laryngeal Transplantation

Cheryl Myers

Head and Neck Regenerative Medicine Laboratory,Center for Regenerative Medicine,Head and Neck Regenerative Medicine Laboratory, Center for Regenerative Medicine

Mayo Clinic Arizona

A Heterotopic Mouse Model for Studying Laryngeal Transplantation

Head and Neck Regenerative Medicine Laboratory,
Center for Regenerative Medicine,
Head and Neck Regenerative Medicine Laboratory, Center for Regenerative Medicine