development-leishmania-species-strains-with-constitutive-expression

Development of <em>Leishmania</em> Species Strains with Constitutive Expression of eGFP

Protozoan parasites of the genus Leishmania cause leishmaniasis, a disease with variable clinical manifestations that affects millions of people ...

Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP)

Chemical examination of the octocoral-associated  species (sp.) DT001 led to the isolation of pumilacidins A () and C (). We investigated the effect of these compounds on the viability of  and the mechanism of pumilacidin-induced death. The use of inhibitors of protein kinase C (PKC) and phosphoinositide 3-kinase (PI3K) was able to prevent the effects of pumilacidins A and C. The results indicated also that pumilacidins inhibit parasite growth via mitochondrial dysfunction and decreased cytosolic Ca.

Pumilacidins from the Octocoral-Associated  sp. DT001 Display Anti-Proliferative Effects in .

Cocos nucifera (C. nucifera) (the coconut palm tree) has been traditionally used to fight a number of human diseases, but only a few studies have tested its components against parasites such as those that cause malaria. In this study, C. nucifera samples were collected from a private natural reserve in Punta Patiño, Darien, Panama. The husk, leaves, pulp, and milk of C. nucifera were extracted and evaluated against the parasites that cause Chagas' disease or American trypanosomiasis (Trypanosoma cruzi), leishmaniasis (Leishmania donovani) and malaria (Plasmodium falciparum), as well as against a line of breast cancer cells. While there was no activity in the rest of the tests, five and fifteen-minute aqueous decoctions of leaves showed antiplasmodial activity at 10% v/v concentration. Removal of some HPLC fractions resulted in loss of activity, pointing to the presence of synergy between the components of the decoction. Chemical molecules were separated and identified using an ultra-performance liquid chromatography (UPLC) approach coupled to tandem mass spectrometry (LC-MS/MS) using atmospheric pressure chemical ionization quadrupole-time of flight mass spectrometry (APCI-Q-TOF-MS) and molecular networking analysis, revealing the presence of compounds including polyphenol, flavone, sterol, fatty acid and chlorophyll families, among others.

Analysis of the antiparasitic and anticancer activity of the coconut palm (Cocos nucifera L. ARECACEAE) from the natural reserve of Punta Patiño, Darién.

In vitro and in vivo testing of new technology was performed to evaluate the antiplasmodial activity of Photonic Multiphase Modulators (PMM) in cultures and in mice previously infected with Plasmodium falciparum and Plasmodium berghei parasites.

Evaluation of the in vitro and in vivo antiplasmodial effect of water treated with Photonic Multiphase Modulators (PMM) designed with Advanced Physics System Engineering (APSE™) and BioPhoton-X™ technology.

Laura Pineda

Centro de Biología Celular y Molecular de Enfermedades

Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP)

Development of Leishmania Species Strains with Constitutive Expression of eGFP