Ian M. Dobbie

Biochemistry,
Micron Advanced Imaging Consortium,
Department of Biochemistry,
Micron Advanced Imaging Consortium, Department of Biochemistry

University of Oxford

BIOGRAPHY

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PUBLICATIONS

Rapid actin transport during cell protrusion.

Science (New York, N.Y.) Apr, 2003 | Pubmed ID: 12677069

Histone H2A phosphorylation and H3 methylation are required for a novel Rad9 DSB repair function following checkpoint activation.

DNA repair Jun, 2006 | Pubmed ID: 16650810

Mobility and distribution of replication protein A in living cells using fluorescence correlation spectroscopy.

Experimental and molecular pathology Apr, 2007 | Pubmed ID: 17303118

Autofluorescent proteins.

Methods in cell biology , 2008 | Pubmed ID: 18155456

Isolation of Drosophila egg chambers for imaging.

Cold Spring Harbor protocols Apr, 2010 | Pubmed ID: 20360356

Collection and mounting of Drosophila embryos for imaging.

Cold Spring Harbor protocols Apr, 2010 | Pubmed ID: 20360357

Drosophila macrophage preparation and screening.

Cold Spring Harbor protocols Apr, 2010 | Pubmed ID: 20360358

Drosophila larval fillet preparation and imaging of neurons.

Cold Spring Harbor protocols Apr, 2010 | Pubmed ID: 20360359

Live cell imaging in Drosophila melanogaster.

Cold Spring Harbor protocols Apr, 2010 | Pubmed ID: 20360379

Signal-dependent turnover of the bacterial flagellar switch protein FliM.

Proceedings of the National Academy of Sciences of the United States of America Jun, 2010 | Pubmed ID: 20498085

OMX: a new platform for multimodal, multichannel wide-field imaging.

Cold Spring Harbor protocols Aug, 2011 | Pubmed ID: 21807861

Remodelling of cortical actin where lytic granules dock at natural killer cell immune synapses revealed by super-resolution microscopy.

PLoS biology Sep, 2011 | Pubmed ID: 21931537

Super-resolution imaging of remodeled synaptic actin reveals different synergies between NK cell receptors and integrins.

Blood Nov, 2012 | Pubmed ID: 22966166

Drosophila patterning is established by differential association of mRNAs with P bodies.

Nature cell biology Dec, 2012 | Pubmed ID: 23178881

Super-resolution microscopy using standard fluorescent proteins in intact cells under cryo-conditions.

Nano letters Jul, 2014 | Pubmed ID: 24884378

A molecular mechanism of mitotic centrosome assembly in Drosophila.

eLife Aug, 2014 | Pubmed ID: 25149451

Assessing resolution in super-resolution imaging.

Methods (San Diego, Calif.) Oct, 2015 | Pubmed ID: 26164701

SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy.

Scientific reports Nov, 2015 | Pubmed ID: 26525406

Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison.

Scientific reports 06, 2016 | Pubmed ID: 27264341

Strategic and practical guidelines for successful structured illumination microscopy.

Nature protocols May, 2017 | Pubmed ID: 28406496

A combined 3D-SIM/SMLM approach allows centriole proteins to be localized with a precision of ∼4-5 nm.

Current biology : CB Oct, 2017 | Pubmed ID: 29017036

SPEKcheck - fluorescence microscopy spectral visualisation and optimisation: a web application, javascript library, and data resource.

Wellcome open research , 2018 | Pubmed ID: 30175248

Bridging the resolution gap: correlative super-resolution imaging.

Nature reviews. Microbiology 06, 2019 | Pubmed ID: 31040390

IsoSense: frequency enhanced sensorless adaptive optics through structured illumination.

Optica Mar, 2019 | Pubmed ID: 31417942

3D Correlative Cryo-Structured Illumination Fluorescence and Soft X-ray Microscopy Elucidates Reovirus Intracellular Release Pathway.

Cell Jul, 2020 | Pubmed ID: 32610083

Implementation of a 4Pi-SMS super-resolution microscope.

Nature protocols 02, 2021 | Pubmed ID: 33328610

Democratising "Microscopi": a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork.

Wellcome open research , 2021 | Pubmed ID: 33977151

Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures.

Nature protocols May, 2021 | Pubmed ID: 33990802

QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy.

Nature methods May, 2021 | Pubmed ID: 34021279

Protocol for image registration of correlative soft X-ray tomography and super-resolution structured illumination microscopy images.

STAR protocols Jun, 2021 | Pubmed ID: 34027487

INSTITUTIONS

University of Oxford

JOVE ARTICLES

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Ian M. Dobbie¹,

Alexander Robson²,

Nicolas Delalez²,

Mark C. Leake²

¹Biochemistry, University of Oxford,

²Physics, University of Oxford

Biology

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Nina Vyas^*,1,

Nina Perry^*,1,

Chidinma A. Okolo¹,

Ilias Kounatidis¹,

Thomas M. Fish¹,

Kamal L. Nahas^1,2,

Archana Jadhav¹,

Mohamed A. Koronfel¹,

Johannes Groen³,

Eva Pereiro³,

Ian M. Dobbie⁴,

Maria Harkiolaki¹

¹Harwell Science and Innovation Campus, Beamline B24, Diamond Light Source,

²Division of Virology, Department of Pathology, University of Cambridge,

³ALBA Synchrotron, Beamline 09 - MISTRAL,

⁴Micron Advanced Imaging Consortium, Department of Biochemistry, University of Oxford

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Ian M. Dobbie

Biochemistry,Micron Advanced Imaging Consortium,Department of Biochemistry,Micron Advanced Imaging Consortium, Department of Biochemistry

University of Oxford

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Biochemistry,
Micron Advanced Imaging Consortium,
Department of Biochemistry,
Micron Advanced Imaging Consortium, Department of Biochemistry