The aim is to assess whether the sensation of exercise performance is due to central or peripheral determinants. Subjects respond differently to viable environmental conditions and intrusive factors. This will be done, using a non-invasive method.
This is near infrared spectroscopy. The new studies in this infrared surgery area, using new technology focus on analyzing brain and massive tissues involved in motor tasks, respiration, and locomotion. Identifying key areas linking to exercise induced fat.
The primary technology used in our study is near infrared spectroscopy, NIS, which allows non-invasive assessment of hematological variables, including oxymyoglobin and deoxymyoglobin, and the regulation of the tissue saturation index. This approach provides a more comprehensive understanding of tissue oxygen utilization. One challenge is to minimize external interference with new signups, such as ambient light and motion artifacts, while ensuring a current placement and calibration of the equipment.
Another could be the variation in participants'anatomy, such as ATT or skin pigmentation, can affect measurement accuracy, regarding standardizations and adjustments during data collections. Our results demonstrate that mirrors can effectively identify central and peripheral exercise limitations by analyzing changes in tissue oxygenation during exercise. This approach highlights how cerebral and muscular responses vary with exercise intensity and offers new ways to optimize training and performance.
To begin, ensure that all wearable devices are fully charged before initiating the placement and measurements. Apply double-sided adhesive tape to all wearable devices to secure them to the participant's skin. Cover each wearable device with a layer of cling film, followed by a layer of waterproof adhesive dressing to protect the devices from sweat.
Then clean the target area using an alcohol pad to remove residue that may interfere with signal registration. Secure the correctly placed wearable devices with a layer of elastic therapeutic tape. Place a black cloth over all wearables to prevent ambient light from penetrating.
Position the NIRS probe on the dorsal lateral prefrontal cortex, approximately 10 millimeters above the participant's superciliary arch. Now, use a B mode ultrasound to confirm NIRS penetration depth by verifying the distance from the subcutaneous tissue to the outer border of the M intercostalis. Then place the NIRS probe over the seventh intercostal space at the right anterior axillary line.
If it cannot be positioned on the right hemithorax, position it on the left hemithorax, but the heart rate signal may be more pronounced on the left side. Measure the skinfold thickness using calipers to confirm that the adipose tissue thickness does not exceed 20 millimeters, ensuring accurate NIRS penetration depth. Place the NIRS probe on the vastus lateralis muscle, positioned five centimeters lateral to the midpoint of the imaginary line, connecting the patellas upper edge and the greater trochanter of the femur.
Once all NIRS wearables are correctly placed, power them on before beginning the measurement. Launch the data acquisition software, provided by the manufacturer. Create a new file for data recording and link all the NIRS wearables.
For muscle measurements, set the differential path length factor to four. Set the sampling rate to 10 hertz for data acquisition and analog to digital conversion for the assessed tissues. Adjust the differential path length factor for prefrontal cortex measurements, according to the participant's age dependent factor.
To begin, open the ergo spirometer software, provided by the manufacturer to begin the calibration process. Using a syringe adapter, attach the flow meter to a 28 millimeter turbine. Connect one corrugated tube to the syringe adapter and the other to a three liter calibration syringe.
Next, perform six withdrawal and injection maneuvers, ensuring a constant flow rate. After completion, the software will automatically confirm if the calibration test has passed. For air calibration, ensure the sample line from the gas analyzer is disconnected from the calibration port and hanging freely before initializing the calibration process.
During calibration, observe a stable flat line, indicating minimal variation in oxygen and carbon dioxide concentrations. Open the gas valves and check the manometer to verify that adequate pressure is being delivered to the system. Connect the sample line to the calibration port and initialize the calibration process.
Then perform a three minute preheating as recommended by the manufacturer before proceeding with calibration. After the three minute preheating period, confirm that two flat lines are observed, one representing room air fluctuations, and the other representing calibration gas levels. Finally, disconnect the sample line from the calibration port and attach it to the mouthpiece for the upcoming test.
Prepare the participant's skin by exfoliating the electrode placement sites with a cream and or shaving any hair, if necessary. Clean the areas with an alcohol pad to remove any superficial tissue residues. Position the bipolar limb lead electrodes, then place the precordial lead electrodes.
After setting up the NIRS devices and calibrating the ergo spirometer, ask the participant to sit on the bike, ensuring that the seat and handlebars are adjusted to their height for optimal comfort and positioning. Explain the test protocol to the participant and instruct the participant to breathe through the mask consistently before, during, and after the test. Attach a fingertip pulse oximeter to the participant's finger.
Once the participant is positioned and prepared, instruct them to extend their right leg and wait for two minutes at the initial rest stage. Begin the warmup phase by having the participant pedal at a cadence of 80 to 100 RPM for six minutes. Gradually, increase the workload at a rate of 20 watts per minute for women and 25 watts per minute for men until the participant reaches exhaustion.
After completing the exercise phase, instruct the participant to remain still and continue breathing into the mask for three minutes during the cool down or recovery phase. Once the exercise protocol is complete, carefully remove the pulse oximeter from the participant's finger, followed by the mask of all three NIRS wearables and the ECG electrodes. In subjects with ventilatory demand during intense exercise, rapid lung ventilation and respiratory rate lead to hyperventilation, causing brain vasoconstriction and thereby, limiting performance by central limitation.
Conversely, in subjects with high locomotor but not respiratory demand, brain vasoconstriction does not occur. Consequently, NIRS data reflects moderate intensity, exercise patterns. In these subjects, exercise performance is limited by peripheral, rather than central limiting factors.
