universidade federal de são paulo

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue

Bruna A. G. de Melo¹, Elisa M. Cruz¹, Taís N. Ribeiro¹, Mayara V. Mundim¹, Marimelia A. Porcionatto¹

¹Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de São Paulo

Here we report a method of 3D bioprinting murine cortical astrocytes for biofabricating neural-like tissues to study the functionality of astrocytes in the central nervous system and the mechanisms involving glial cells in neurological diseases and treatments.

Immunology and Infection

Demystifying In Vivo Bioluminescence Imaging of a Chagas Disease Mouse Model for Drug Efficacy Studies

Adriana C. da Silva¹, Jadel M. Kratz², Priscylla G. M. Morgado^1,3, Lucio H. Freitas-Junior¹, Carolina B. Moraes^1,4

¹Departamento de Microbiologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo, ²Drugs for Neglected Diseases initiative (DNDi) Latin America, ³Programa de Pós-Graduação em Microbiologia e Imunologia, Universidade Federal de São Paulo, ⁴Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo

The protocol provided here describes the detailed steps to perform drug efficacy studies in a bioluminescent model of Trypanosoma cruzi infection, with a focus on data acquisition, analysis, and interpretation. Troubleshooting and quality control procedures to minimize technical issues are also provided.