In Vivo Whole-Brain Imaging of Zebrafish Larvae Using Three-Dimensional Fluorescence MicroscopyEun-Seo Cho 1, Seungjae Han 1, Gyuri Kim 1, Minho Eom 1, Kang-Han Lee 2, Cheol-Hee Kim 2, Young-Gyu Yoon 1,3
1School of Electrical Engineering, KAIST, 2Department of Biology, Chungnam National University, 3KAIST Institute for Health Science and Technology
Presented here is a protocol for in vivo whole-brain imaging of larval zebrafish using three-dimensional fluorescence microscopy. The experimental procedure includes sample preparation, image acquisition, and visualization.