University of Bordeaux

2 ARTICLES PUBLISHED IN JoVE

Medicine

Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement

Alban Longchamp^*1, Florent Allagnat^*2, Xavier Berard³, Florian Alonso², Jacques-Antoine Haefliger², Sébastien Deglise^*4, Jean-Marc Corpataux^*4

¹Department of Surgery, Brigham and Women's Hospital/Harvard Medical School, ²Laboratory of Experimental Medicine, Department of Medicine, CHUV University Hospital, ³Department of Vascular Surgery, Pellegrin Hospital, University of Bordeaux, ⁴Department of Thoracic and Vascular Surgery, CHUV University Hospital

The mechanisms leading to the development of intimal hyperplasia (IH) and vein graft failure are still poorly understood. This study describes an ex vivo system to perfuse human veins under controlled flow and pressure. Furthermore the efficiency of external mesh reinforcement to limit the development of IH was evaluated.

Neuroscience

Assessing Pupil-linked Changes in Locus Coeruleus-mediated Arousal Elicited by Trigeminal Stimulation

Maria Paola Tramonti Fantozzi^*1, Tommaso Banfi^*2,3, Vincenzo De Cicco^*1, Massimo Barresi⁴, Enrico Cataldo⁵, Davide De Cicco¹, Luca Bruschini⁶, Paola d'Ascanio¹, Gastone Ciuti^2,3, Ugo Faraguna^1,7, Diego Manzoni¹

¹Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, ²The BioRobotics Institute, Scuola Superiore Sant'Anna, ³Department of Excellence in Robotics & AI, Scuola Superiore Sant'Anna, ⁴Institut des Maladie Neurodegeneratives, University of Bordeaux, ⁵Department of Physics, University of Pisa, ⁶Department of Surgical, Medical, Molecular Pathology and Critical Care Medicine, University of Pisa, ⁷Department of Developmental Neuroscience, IRCCS Foundation Stella Maris

To verify whether trigeminal effects on cognitive performance involve locus coeruleus activity, two protocols are presented that aim to evaluate possible correlations between the performance and task-related pupil size changes induced by chewing. These protocols may be applied to conditions in which locus coeruleus contribution is suspected.