Identification of Peptides of Small Extracellular Vesicles from Bone Marrow-Derived MacrophagesJiale Cheng 1,2, Jiong Zhu 1,2, Yujiao Liu 2,3, Chen Yang 2,3, Yuehui Zhang 2, Yuchen Liu 2, Chaozhi Jin 2, Jian Wang 1,2
1School of Basic Medical Sciences, Anhui Medical University, 2State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, 3School of Life Sciences, Hebei University
This protocol describes a procedure to isolate small extracellular vesicles from macrophages by differential ultracentrifugation and extract the peptidome for identification by mass spectrometry.