A Validatable Droplet Digital Polymerase Chain Reaction Assay for the Detection of Adeno-Associated Viral Vectors in Bioshedding Studies of TearsBryanna Longacre 1, Amanda Souaysene 1, Carrie A. Vyhlidal 1, Matthew R. Pennington 1
1Cell and Gene Therapy, KCAS Bioanalytical and Biomarker Services
Here, we present a protocol for the development and validation of good laboratory practices in the compliant detection of adeno-associated viral vectors in human tears by droplet digital polymerase chain reaction in support of clinical development of gene therapy vectors.
