Alma Mater Studiorum-University of Bologna

1 ARTICLES PUBLISHED IN JoVE

Biology

Basic Three-Dimensional (3D) Intestinal Model System with an Immune Component

Francesca Truzzi¹, Silvia Dilloo¹, Xinyue Chang¹, Anne Whittaker¹, Eros D'Amen¹, Giovanni Dinelli¹

¹Department of Agricultural and Food Sciences, Alma Mater Studiorum-University of Bologna

Here we describe constructing a basic three-dimensional (3D) intestinal cell line model system and a paraffin embedding protocol for light microscopic evaluation of fixed intestinal equivalents. Staining of selected proteins permits the analysis of multiple visual parameters from a single experiment for potential use in preclinical drug screening studies.