Three-Dimensional Bioprinting of Human iPSC-Derived Neuron-Astrocyte Cocultures for Screening ApplicationsChloe Ann Whitehouse 1, Yufang He 2, Janet Brownlees 1, Nicola Corbett 1
1MSD Research Laboratories, London, UK, 2Merck & Co., Inc., Rahway, NJ, USA
Here, we present a protocol to produce 3D-bioprinted cocultures of iPSC-derived neurons and astrocytes. This coculture model, generated within a hydrogel scaffold in 96- or 384-well formats, demonstrates high post-print viability and neurite outgrowth within 7 days and shows the expression of maturity markers for both cell types.
