Isolation of Pure Astrocytes and Microglia from the Adult Mouse Spinal Cord For In Vitro Assays and Transcriptomic StudiesJulie J. Ahn 1, Robert H. Miller 1, Yusra Islam 1
1Department of Anatomy and Cell Biology, The George Washington University School of Medicine
This protocol outlines the isolation of purified astrocytes and microglia from the adult mouse spinal cord, facilitating subsequent applications such as RNA analysis and cell culture. It includes detailed cell dissociation methods and procedures designed to enhance both the quality and yield of isolated cells.
