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Chang Gung Memorial Hospital

7 ARTICLES PUBLISHED IN JoVE

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Medicine

Assessment of Right Ventricular Structure and Function in Mouse Model of Pulmonary Artery Constriction by Transthoracic Echocardiography
Hui-Wen Cheng *1,2, Sudeshna Fisch *1, Susan Cheng 1, Michael Bauer 1, Soeun Ngoy 1, Yiling Qiu 1, Jian Guan 1, Shikha Mishra 1, Christopher Mbah 1, Ronglih Liao 1
1Cardiac Muscle Research Labratory, Cardiovascular Division, Brigham and Women’s Hospital, Harvard Medical School, 2Cardiovascular Department, Chang Gung Memorial Hospital

Right ventricle (RV) dysfunction is critical to the pathogenesis of cardiovascular disease, yet limited methodologies are available for its evaluation. Recent advances in ultrasound imaging provide a noninvasive and accurate option for longitudinal RV study. Herein, we detail a step-by-step echocardiographic method using a murine model of RV pressure overload.

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Bioengineering

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
Yen-Li Wang 1,2, Adrienne Hong 3, Tzung-Hai Yen 1,4,5,6, Hsiang-Hsi Hong 1,7,8
1Chang Gung University, 2Department of Periodontics, Chang Gung Memorial Hospital, 3California Northstate University College of Medicine, 4Department of Nephrology, Clinical Poison Center, Chang Gung Memorial Hospital, 5Kidney Research Center, Chang Gung Memorial Hospital, 6Center for Tissue Engineering, Chang Gung Memorial Hospital, 7Department of Periodontics, Chang Gung Memorial Hospital, 8College of Oral Medicine, Taipei Medical University

We present a protocol to investigate the mRNA expression biomarkers of periosteum-derived cells (PDCs) induced by vitamin C (vitamin C) and 1,25-dihydroxy vitamin D [1,25-(OH)2D3]. In addition, we evaluate the ability of PDCs to differentiate into osteocytes, chondrocytes, and adipocytes.

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Medicine

Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery
Shao-Chieh Chiu 1, Shih-Ting Hsu 1, Chiun-Wei Huang 1, Wu-Chung Shen 2, Shin-Lei Peng 2
1Center for Advanced Molecular Imaging and Translation, Chang Gung Memorial Hospital, 2Department of Biomedical Imaging and Radiological Science, China Medical University

The overall goal of this procedure is to measure the blood flow in the rat common carotid artery by using noninvasive phase contrast magnetic resonance imaging.

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Neuroscience

Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release
Ya-Tin Lin 1, Jin-Chung Chen 1,2,3
1Graduate Institute of Biomedical Sciences, Department of Physiology and Pharmacology, Chang Gung University, 2Healthy Aging Research Center, Chang Gung University, 3Neuroscience Research Center, Chang Gung Memorial Hospital

Dorsal root ganglia (DRG) primary cultures are frequently used to study physiological functions or pathology-related events in sensory neurons. Here, we demonstrate the use of lumbar DRG cultures to detect the release of neurotransmitters after neuropeptide FF receptor type 2 stimulation with a selective agonist.

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Bioengineering

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
Yueh-Feng Wu 1, Rai-Teng Ye 1, Ming-Kai Pan 2,3, Sung-Jan Lin *1,3,4,5, Hsin-Yuan Tan *6,7
1Department of Biomedical Engineering, National Taiwan University, 2Institute of Pharmacology, College of Medicine, National Taiwan University, 3Molecular Imaging Center, National Taiwan University, 4Department of Dermatology, National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 6Department of Ophthalmology, Chang Gung Memorial Hospital, 7College of Medicine, Chang Gung University

Presented here is a multiphoton microscopic platform for live mouse ocular surface imaging. Fluorescent transgenic mouse enables the visualization of cell nuclei, cell membranes, nerve fibers and capillaries within the ocular surface. Non-linear second harmonic generation signals derived from collagenous structures provide label-free imaging for stromal architectures.

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Medicine

Development of a Novel Task-oriented Rehabilitation Program using a Bimanual Exoskeleton Robotic Hand
Yi-Mei Chen 1, Szu-Shen Lai 1, Yu-Cheng Pei 2,3,4,5, Chia-Ju Hsieh 1, Wei-Han Chang 1
1Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Taoyuan, 2Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Linkou, 3School of Medicine, Chang Gung University, 4Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital, 5Healthy Aging Research Center, Chang Gung University

This study reports the development of a novel robot-assisted task-oriented program for hand rehabilitation. The developmental process consists of experiments using both healthy subjects and subjects who have had a stroke and suffered from subsequent motor control dysfunction.

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Cancer Research

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
Chun-Chia Cheng 1, Po-Jui Hsu 4, Zong-Lin Sie 1, Fang-Hsin Chen 1,2,3
1Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou, 2Department of Medical Imaging and Radiological Sciences, Chang Gung University, 3Department of Radiation Oncology, Chang Gung Memorial Hospital, 4Department of Laboratory Medicine, Mackay Memorial Hospital

Presented here is a protocol to discover the overexpressed driver genes maintaining the established cancer stem-like cells derived from colorectal HT29 cells. RNAseq with available bioinformatics was performed to investigate and screen gene expression networks for elucidating a potential mechanism involved in the survival of targeted tumor cells.

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