Sign In

Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology

1 ARTICLES PUBLISHED IN JoVE

image

Biochemistry

Cryo-Electron Microscopy Screening Automation Across Multiple Grids Using Smart Leginon
Anjelique Sawh-Gopal 1,2, Aygul Ishemgulova *1, Eugene Y. D. Chua *1, Mahira F. Aragon *1, Joshua H. Mendez 1, Edward T. Eng 1, Alex J. Noble 1
1Simons Electron Microscopy Center, New York Structural Biology Center, 2Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology

Cryo-electron microscopy (cryoEM) multi-grid screening is often a tedious process that demands hours of attention. This protocol shows how to set up a standard Leginon collection and Smart Leginon Autoscreen to automate this process. This protocol can be applied to the majority of cryoEM holey foil grids.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved