Qilu University of Technology (Shandong Academy of Sciences)

1 ARTICLES PUBLISHED IN JoVE

Biology

Rearing Axenic Delia antiqua with Half-Fermented Sterile Diets

Xin Cao¹, Qingxia Liang¹, Miaomiao Li¹, Xiaoqing Wu¹, Susu Fan¹, Xinjian Zhang¹, Fangyuan Zhou¹, Zhiqing Zhao²

¹Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), ²Agriculture and Rural Bureau of Changqing District

A simple procedure for rearing axenic Delia antiqua with half-fermented sterile diets is described. Only one Wolbachia strain was detected in each instar of axenic D. antiqua using PCR.