Isolating Interaction-Null/Impaired Mutants Using the Yeast Two-Hybrid AssayYuna Satake 1, Nozomi Gotouda 1, Seiji Tanaka 1,2
1Graduate School of Engineering, Kochi University of Technology, 2School of Engineering Science, Kochi University of Technology
The interactions of biomolecules, such as protein-protein interactions, are the molecular basis of biological functions. If interaction-null/impaired mutants that specifically lack the relevant interaction can be isolated, they will greatly help to understand the function(s) of this interaction. This article presents an efficient way to isolate interaction-null/impaired mutants.
