CURRICULUM VITAE

Name: Ning Li

Present Status: Professor

Present address: Division of Life Science

The Hong Kong University of Science and Technology

Clear Water Bay, Kowloon, Hong Kong SAR.

Tel: 852-358-7335;

Fax: 852-358-1559.

E-mail: boningli@.ust.hk

Website http://life-sci.ust.hk/team/ning-li/

Education

1989 Ph. D University of Washington, Seattle, WA. USA

Subject area : plant molecular biology and biochemistry

Professional positions

1993 – present Professor, Associate Professor and Assistant Professor

Division of Life Science, HKUST, Hong Kong SAR, China.

1989 – 1993 Research Associate, USDA/ARS/PSI, Plant Molecular Biology Laboratory, Beltsville, MD. USA.

1989 Postdoctoral Fellow, Department of Biochemistry, University of Washington, Seattle, WA. USA.

Honorary positions

2017 – present Professor of Energy Institute, and Institute for the Environment, HKUST

2013 – present Professor of Shenzhen Research Institute, HKUST

2016 – 2019 Adjunct Senior Research Fellow of Chinese Academy of Tropical Agricultural Science

2012-2013 Chair of Gordon Research Conference on the PTM network

2011 Director of the Croucher Advanced Study Institute

2010-2012 Director of CAS-Croucher and HKUST Joint lab

2006 – 2011 Adjunct Professor of Lanzhou University, Lanzhou, China.

2005 Scientist in Salk Institute for Biological Studies, San Diego, USA.

1999-present Adjunct Senior Research Fellow of Institute of Botany, Chinese Academy of Sciences, Beijing, China

2001- present Adjunct Senior Research Fellow of Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.

2001- present Adjunct Senior Research Fellow of Jiangshu Institute of Botany and Zhong Shan Botanic Garden, Chinese Academy of Sciences, Nanjing, China.

2001- 2005 Adjunct Professor of Zhongshan University, Guangzhou, China.

Research Achievements

1. Establishment and development of MS-based quantitative in vivo interactomics, IPQCX-MS.

2. Establishment of dimethyl-labeling-based quantitative PTM proteomics approach and discovery of ethylene-regulated acetylproteome.

3. Establishment of SILIA-based quantitative PTM proteomic and molecular genetic analysis of Arabidopsis touch response (thigmomorphogenesis) and discovery of TREPHs (touch-regulated phosphoproteins) playing a key role in plant mechano-signaling.

4. Functional and quantitative PTM proteomics analysis of EIN2- and EIN3/EIL1-independent phosphor-relay pathways and discovery of the dual-and-opposing (Yin-Yang) mode of action for ethylene regulation as well as the roles of phosphorylated water channel protein PIP2;1 isoform and Ser62-phosphorylated ERF110 in ethylene response.

5. Identification of novel ethylene-regulated time-dependent phosphoproteins and putative CTR1 substrates using SILIA-based quantitative and differential phosphoproteomics.

6. Establishment of SILIA-based absolute quantitation of isoform of post-translationally modified redox proteins (OxNSIL), which will provide practical means to study the redox state on redox-regulated PTM proteins.

7. Establishment of SILIA-based absolute quantitation of isoform of post-translationally modified (PTM) proteins (AQUIP), which will provide practical means to study “combinatorial PTM code” on PTM proteins.

8. Establishment of 15N stable isotope labeling in Arabidopsis (SILIA) protocol for in vivo quantitation of protein PTM, which will provide a large scale of high-throughput data for construction of phosphor-relay-mediated cell signaling network in plants.

9. Discovery of cis-cinnamic acid (cis-form) being a new class of growth regulator affecting many aspects of plant growth and development ranging from seed germination, seedling development, flowering and fruit ripening, leading to the molecular cloning of cis-cinnamate-inducible genes (ZCE1) using label-free differential proteomics approach and the map-based cloning of a cis-phenylpropanoid response (CPAR) genes.

10. Molecular breeding of transgenic citrus plants over-expressing chilling-inducible antisense ACC synthase gene.

11. The application of ethylene as a novel gaseous reporter in animal cells.

12. Improvement of tomato fruit quality through genetic engineering of SAM decarboxylase.

A. Five most representative journal publications in recent 5 years

1. Wang, K., Yang, Z., Qing, D., Ren, F., Liu, S., Zheng, Q., Liu, J., Zhang, W., Dai, C., Wu, M., Chehab, E.W., Braam, J., Li, Ning*. (2018). Quantitative and functional posttranslational modification proteomics reveals that TREPH1 plays a role in plant touch-delayed bolting. Proceedings of the National Academy of Sciences. 115, 115, 10830-10835.

2. Liu, SC, Yu, FC, Yang Z, Wang T, Xiong, H Chang, C, Yu, WC*, and Ning Li* (2018) Establishment of dimethyl labelling-based quantitative acetylproteomics in Arabidopsis. Mol Cell Proteomics. 17, 1010-1027.

3. Zhu, XL, Yu, FC, Yang, Zhu, Liu, SC, Dai C, Lu, XY, Liu, CY, Yu, WC* and Ning Li* (2016) In planta Chemical Cross-linking and Mass Spectrometry Analysis of Protein Structure and Interaction in Arabidopsis. Proteomics. 16, 1915-1927.

4. Liu, SC, Yu, FC, Hu, Q, Wang, TL, Yu, LJ, Du, SW, Yu, WC* and Ning Li*. (2018) Development of in Planta Chemical Cross-Linking-Based Quantitative Interactomics in Arabidopsis. J. Proteome Research. 17, 3195-3213.

5. Zhu Yang, Guangyu Guo, Manyu Zhang, Claire Y. Liu, Qin Hu, others and Ning Li* (2013) Stable Isotope Metabolic Labeling-Based Quantitative Phosphoproteomic Analysis of Arabidopsis Mutants Reveals Ethylene-Regulated Time-Dependent Phosphoproteins and Putative Substrates of CONSTITUTIVE TRIPLE RESPONSE 1 Kinase. Molecular and Cellular Proteomics. 12: 3559-3582.

B. Five representative journal publications beyond the recent 5 years

1. Zhu, L; Liu, D; Li, Y and others, Ning Li* (2013) Functional Phosphoproteomic Analysis Reveals That a Serine-62-Phosphorylated Isoform of Ethylene Response Factor110 Is Involved in Arabidopsis Bolting. Plant Physiology. 161, 904-917.

2. Li, H, Wong WS, Guo, HW, Ecker, J and Ning Li* (2009) Proteomics. 9, 1646-1661.

3. Chen, G, Bi YR and Ning Li (2005) The Plant Journal. 41, 363-375.

4. Mehta, RA,Cassol, T, Li,Ning, Ali,N , Handa,AK and Mattoo, AK (2002) Nature Biotechnology. 20, 613-618.

5. Huai Q , others, Li, Ning and Ke, HM . (2001). J. Biol. Chem. 276, 38210-38216.

Regeneron Pharmaceuticals Inc.

1 ARTICLES PUBLISHED IN JoVE

Chemistry