Instituto de Investigaciones Científicas y Servicios de Alta Tecnología AIP (INDICASAT AIP)

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions

Héctor Cruz^1,2, Alejandro Llanes^2,3, Patricia L. Fernández^2,3

¹Facultad de Ciencias y Tecnología, Universidad Tecnológica de Panamá (UTP), ²Centro de Biología Molecular y Celular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología AIP (INDICASAT AIP), ³Sistema Nacional de Investigación de Panamá (SNI)

We describe a methodology based on sequence diversification to estimate the amino acid preferences of multispecific binding sites in protein-protein interactions (PPIs). In this strategy, thousands of potential peptide ligands are generated and screened in silico, thus overcoming some limitations of available experimental methods.