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Dalian Medical University

7 ARTICLES PUBLISHED IN JoVE

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Medicine

A Novel Murine Model of Arteriovenous Fistula Failure: The Surgical Procedure in Detail
Chun Yu Wong 1,2,3, Margreet R. de Vries 2,3, Yang Wang 4, Joost R. van der Vorst 3, Alexander L. Vahrmeijer 3, Anton-Jan van Zonneveld 1,2, Jaap F. Hamming 3, Prabir Roy-Chaudhury 4, Ton J. Rabelink 1,2, Paul H. A. Quax 2,3, Joris I. Rotmans 1,2
1Department of Nephrology, Leiden University Medical Center, 2Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, 3Department of Surgery, Leiden University Medical Center, 4Division of Nephrology, University of Cincinnati

Here we present a murine model of arteriovenous fistula (AVF) failure in which a clinically relevant anastomotic configuration is incorporated. This model can be used to study the pathophysiology and to test possible therapeutic interventions.

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Genetics

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
Huan-Huan Wei *1, Yuanlong Liu *1, Yang Wang 2, Qianyun Lu 1, Xuerong Yang 1, Jiefu Li 1, Zefeng Wang 1
1Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences (SIBS), 2Institute of Cancer Stem Cell, Second Affiliated Hospital, Cancer Center, Dalian Medical University

This report describes a bioengineering method to design and construct novel Artificial Splicing Factors (ASFs) that specifically modulate the splicing of target genes in mammalian cells. This method can be further expanded to engineer various artificial factors to manipulate other aspects of RNA metabolism.

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JoVE Journal

Rapid Repetition Rate Fluctuation Measurement of Soliton Crystals in a Microresonator
Peng Xie *1,2, Xinyu Wang *1,2, Weiqiang Wang 1,2, Wenfu Zhang 1,2, Zhizhou Lu 1,2, Yang Wang 1,2, Wei Zhao 1,2
1State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics (XIOPM), Chinese Academy of Sciences (CAS), 2University of Chinese Academy of Sciences

Here, we present a protocol to generate soliton crystals in a butterfly-packaged micro-ring resonator using a thermal tuned method. Further, the repetition rate fluctuations of a soliton crystal with a single vacancy are measured using a delayed self-heterodyne method.

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Developmental Biology

Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
Yang Wang 1, Arun Sampathkumar 1
1Max Planck Institute of Molecular Plant Physiology

Here we describe a protocol for live cell imaging of the cortical microtubule cytoskeleton at the shoot apical meristem and monitoring its response to changes in physical forces.

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Biology

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
Yiling Yang *1, Qianye Chen *2, Siru Zhou 1, Xinyi Gong 1, Hongyuan Xu 1, Yueyang Hong 1, Qinggang Dai 3, Lingyong Jiang 1
1Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology, 2Department of Stomatology, Dalian Medical University, 3The 2nd Dental Center, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology

This protocol describes a canonical method to understand the critical genes controlling osteoclast activity in vivo. This method uses a transgenic mouse model and some canonical techniques to analyze skeletal phenotype.

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Medicine

Generation and Characterization of Right Ventricular Myocardial Infarction Induced by Permanent Ligation of the Right Coronary Artery in Mice
Ruoxi Liao 1, Mingyuan He 2, Donghong Hu 2, Chengzheng Gong 1, Huiying Du 1, Hairuo Lin 2, Huijun Sun 3
1The First Clinical College of Dalian Medical University, 2Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, 3College of Pharmacy, Dalian Medical University

There are several differences between the right and left ventricles. However, the pathophysiology of right ventricular infarction (RVI) has not been clarified. In the present protocol, a reproducible method for RVI mouse model generation is introduced, which may provide a means to explain the mechanism of RVI.

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Chemistry

A Scalable Balz-Schiemann Reaction Protocol in a Continuous Flow Reactor
Zhangtao Zhou 1,2, Yang Wang 1, Shuqiang Liu 1, Andrew Phillis 1,2, Qijun Zhang 1, Weiping Ye 1
1Shenzhen Hwagen Pharmaceutical Co., Ltd, 2Guangdong Raffles Pharmaceutical Technology Co., Ltd

A detailed scalable continuous flow protocol is presented to synthesize an aryl fluoride from an aryl amine through the Balz-Schiemann reaction.

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