Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In VitroCraig McBeth 1, David Brown 2, Pawel Pokorski 2, Lydia Lei 2, Vicki Stone 2
1School of Health and Life Sciences, Teesside University, 2Institute of Biochemical Chemistry, Biophysics, and Bioengineering, Heriot-Watt University
Quantification of both oxidized and reduced forms of glutathione (GSSG and GSH, respectively) has been achieved through the use of Ortho-phthalaldehyde (OPA). OPA becomes highly fluorescent once conjugated to GSH but is unable to conjugate GSSG until reduced. Here, we describe a multiparametric assay to quantify both using protein quantification for normalization.
