Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide GelsNadine Dyballa 1, Sabine Metzger 1
1Biological Medical Research Center (BMFZ), University Duesseldorf
This video shall popularize a colloidal Coomassie G-250 staining protocol according to Kang et al. for the detection of average 4 ng protein in gels. The staining is completed within 2 hours and without any effort. We routinely use Kang's protocol for analytical purposes in gel-based proteomics.
