University of Texas San Antonio

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Intratracheal Inoculation of Fischer 344 Rats with Francisella tularensis

Jesse Q. Nguyen¹, Xhavit Zogaj¹, Aanuoluwa A. Adelani¹, Ping Chu², Jieh-Juen Yu¹, Bernard P. Arulanandam¹, Karl E. Klose¹

¹South Texas Center for Emerging Infectious Diseases, University of Texas San Antonio, ²Midwestern University

This protocol describes intratracheal inoculations of Fischer 344 rats with Francisella tularensis. This procedure mimics pulmonary exposure of humans to this potential biothreat agent and can be used to test vaccine and therapeutic efficacy against pulmonary tularemia.