Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in DrosophilaHiroshi Ishimoto 1, Hiroko Sano 2
1Division of Biological Science, Graduate School of Science, Nagoya University, 2Department of Molecular Genetics, Institute of Life Science, Kurume University
This paper describes a protocol for ex vivo calcium imaging of the Drosophila brain. In this method, natural or synthetic compounds can be applied to the buffer to test their ability to activate particular neurons in the brain.