Tel-Aviv Sourasky Medical Center

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Spatial High-resolution Analysis of Gene Expression Levels in Tendons

Steffany Villaseñor¹, Mor Grinstein^1,2,3

¹Center for Regenerative Medicine, Department of Orthopedic Surgery, Massachusetts General Hospital, Harvard Medical School, ²Harvard Stem Cell Institute, ³Center Of Regeneration and Longevity (CORAL), Tel-Aviv Sourasky Medical Center

This article describes how to perform an optimized in situ protocol for tendons. This method discusses tissue preparation, section permeabilization, probe design, and signal amplification methods.

Developmental Biology

Two-Photon Microscopy for the Study of Tendons

Steffany Villaseñor¹, Mor Grinstein^1,2,3

This article outlines the process of preparing, setting up, and imaging tendons using multiphoton microscopy. Additionally, it covers the application of SHG for analyzing collagen fibril alignment and the creation of a 3D representation of tendons. This methodology proves highly valuable in characterizing tendon cells and their ECM during injury and development.