Ecole Polytechnique Fédérale Lausanne

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Veronika Cencen¹, Bahareh Ghadiani¹, Santiago H. Andany¹, Mustafa Kangül¹, Cem Tekin², Marcos Penedo¹, Maartje Bastings², Georg E. Fantner¹

¹Laboratory for Bio-Nano Instrumentation, Interfaculty Bioengineering Institute, School of Engineering, Ecole Polytechnique Fédérale Lausanne, ²Programmable Biomaterials Laboratory, Institute of Materials, School of Engineering, Ecole Poly-technique Fédérale Lausanne

Here, we show the imaging protocol for observing biomolecular interactions with photothermal off-resonance tapping (PORT), where we optimized imaging parameters, identified system limits, and investigated potential improvements in imaging three-point-star DNA motif assembly.