National Institute of Science Education and Research

1 ARTICLES PUBLISHED IN JoVE

Biology

Fission Yeast as a Platform for Antibacterial Drug Screens Targeting Bacterial Cytoskeleton Proteins

Sakshi Mahesh Poddar^1,2, Srijita Roy^1,2, Ajay Kumar Sharma^1,2, Ramanujam Srinivasan^1,2

¹School of Biological Sciences, National Institute of Science Education and Research, ²Homi Bhabha National Institutes

Fission yeast is used here as a heterologous host to express bacterial cytoskeletal proteins such as FtsZ and MreB as translational fusion proteins with GFP to visualize their polymerization. Also, compounds that affect polymerization are identified by imaging using a fluorescence microscope.