Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug DiscoveryIsotta Sturniolo 1,2, Csongor Váróczy 1,3, Ákos Máté Bede 1,3, Csaba Hegedűs 1, Máté Á. Demény 1,4, László Virág 1,4
1Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, 2Doctoral School of Molecular Medicine, University of Debrecen, 3National Academy of Scientist Education, 4HUN-REN-DE Cell Biology and Signaling Research Group
Here, we present a method to identify compounds that modulate the ADCC mechanism, an important cancer cell-killing mechanism of antitumor antibodies. The cytotoxic effect of NK cells is measured in breast cancer cell spheroids in the presence of Trastuzumab. Image analysis identifies live and dead killer and target cells in spheroids.