Tsinghua Shenzhen International Graduate School

1 ARTICLES PUBLISHED IN JoVE

Biology

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Changyue Liu^*1,2, Zhengyang Lei^*1,2, Lijin Lian², Likun Zhang^1,2, Zhicheng Du^1,2, Peiwu Qin^1,2

¹Center of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, ²Institute of Biopharmaceutics and Health Engineering, Tsinghua Shenzhen International Graduate School

We present a protocol that combines recombinase polymerase amplification with a CRISPR/Cas12a system for trace detection of DNA viruses and builds portable smartphone microscopy with an artificial intelligence-assisted classification for point-of-care DNA virus detection.