Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the RescueSara Bertuzzi 1, Ana Poveda 1, Ana Ardá 1,2, Ana Gimeno 1,2, Jesús Jiménez-Barbero 1,2,3
1CIC bioGUNE, Basque Research and Technology Alliance (BRTA), 2lkerbasque, Basque Foundation for Science, 3Centro de Investigacion Biomedica En Red de Enfermedades Respiratorias
Here, we present a protocol detailing the acquisition, processing, and analysis of a series of NMR experiments aimed at characterizing protein-glycan interactions in solution. Most common ligand-based and protein-based methodologies are outlined, which undoubtedly contribute to the fields of structural glycobiology and molecular recognition studies.
