Centro de Investigacion Biomedica En Red de Enfermedades Respiratorias

1 ARTICLES PUBLISHED IN JoVE

Chemistry

Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the Rescue

Sara Bertuzzi¹, Ana Poveda¹, Ana Ardá^1,2, Ana Gimeno^1,2, Jesús Jiménez-Barbero^1,2,3

¹CIC bioGUNE, Basque Research and Technology Alliance (BRTA), ²lkerbasque, Basque Foundation for Science, ³Centro de Investigacion Biomedica En Red de Enfermedades Respiratorias

Here, we present a protocol detailing the acquisition, processing, and analysis of a series of NMR experiments aimed at characterizing protein-glycan interactions in solution. Most common ligand-based and protein-based methodologies are outlined, which undoubtedly contribute to the fields of structural glycobiology and molecular recognition studies.