A Versatile Pipeline for Analyzing Dynamic Changes in Nuclear Bodies in a Variety of Cell TypesValeria Di Gioia 1,2, Jan Zamporlini 1,3, Rebecca Vadalà 1,3, Elena Parmigiani 4, Beatrice Bodega 1,3, Federica Marasca 1
1Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi" (INGM), 2SEMM, European School of Molecular Medicine, 3Department of Biosciences, University of Milan, 4T-One Therapeutics Srl
This method describes an immunofluorescence protocol and quantification pipeline for evaluating protein distribution with varied nuclear organization patterns in human T lymphocytes. This protocol provides step-by-step guidance, starting from sample preparation and continuing through the execution of semi-automated analysis in Fiji, concluding with data handling by a Google Colab notebook.
