ArcticZymes Technologies ASA

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Ronja Stelzer¹, Elizabeth Rzoska-Smith¹, Sigurd Gundesø², Ulli Rothweiler², Adele Williamson¹

¹University of Waikato, ²ArcticZymes Technologies ASA

Here, a protocol for assaying nucleic acid metabolizing enzymes is presented, using examples of ligase, nuclease, and polymerase enzymes. The assay utilizes fluorescently labeled and unlabeled oligonucleotides that can be combined to form duplexes mimicking RNA and/or DNA damages or pathway intermediates, allowing for the characterization of enzyme behavior.