Identification of Quiescent Cells in a Zebrafish T-Cell Acute Lymphoblastic Leukemia Model Using Cell Proliferation Staining Majd A. Al-Hamaly 1,2, Yelena Chernyavskaya 3, Jessica S. Blackburn 2,3
1Pharmacology and Nutritional Sciences, The University of Kentucky, 2Markey Cancer Center, The University of Kentucky, 3Department of Molecular and Cellular Biochemistry, The University of Kentucky
We used cell proliferation staining to identify quiescent cells in the zebrafish T-acute lymphoblastic leukemia model. The stain is retained in non-dividing cells and reduced during cell proliferation, enabling the selection of dormant cells for further interrogation. This protocol provides a functional tool to study self-renewal in the context of cellular quiescence.
