Preliminary Validation of Stereotaxic Injection Coordinates via CryosectioningXiaoxin Zhou *1,2,3, Wanbing Dai *1,2, Jie Zhou *1,2, Yizhe Zhang 1,2, Xiao Zhang 1,2, Sihan Chen 1,2, Xiaoyu Sun 1,2
1Department of Anesthesiology, Renji Hospital, Shanghai Jiaotong University, School of Medicine, 2Key Laboratory of Anesthesiology (Shanghai Jiaotong University), Ministry of Education, 3Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiaotong University
The present protocol describes a practical strategy to expedite the verification step of stereotaxic injection coordinates before conducting viral tracing using dyes and frozen sections.