Hackensack Meridian Health

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice

Michael T. McGuire^*1, Andrea Z. Tuckett^*2, Faith Myint², Johannes L. Zakrzewski^2,3,4

¹Department of Radiology, Hackensack University Medical Center, ²Center for Discovery and Innovation, Hackensack Meridian Health, ³Department of Pediatrics, Hackensack University Medical Center, ⁴Department of Oncology, Georgetown University

The present protocol describes an interventional radiology procedure established for intrathymic injection in mice to avoid the risk of open surgery and improve the accuracy of blind percutaneous injections.

Biology

Preparation of Agar Bead Embedded Mycobacterium abscessus to Inoculate Immunocompetent Mice Intratracheally

Julianna Proietto^*1, Betelhem Tatek^*1, Camilla Folvar^*1, Diana C. Jimenez², Ilham M. Alshiraihi^2,3, Mercedes Gonzalez-Juarrero², Fabio Saliu⁴, Federica Vacca⁴, Daniela Maria Cirillo⁴, Nicolà Lore⁴, Thomas Dick^1,5,6, Véronique Dartois^1,5, Wassihun Wedajo Aragaw¹

¹Center for Discovery and Innovation, Hackensack Meridian Health, ²Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, ³Department of Biological Sciences, College of Science, University of Tabuk, ⁴Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, ⁵Department of Medical Sciences, Hackensack Meridian School of Medicine, ⁶Department of Microbiology and Immunology, Georgetown University

Mice are generally resistant to infections by Mycobacterium abscessus, which complicates the discovery and development of much-needed antibiotics against pulmonary infections. Here, we describe a method of inoculum preparation and intratracheal infection that was shown to deliver sustained infection in immunocompetent mice.