the first affiliated hospital of Sun Yat-sen University

6 ARTICLES PUBLISHED IN JoVE

Cancer Research

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

Ruoji Zhou^1,2, An Xu¹, Jian Tu^1,3, Mo Liu¹, Julian A Gingold⁴, Ruiying Zhao¹, Dung-Fang Lee^1,2,5,6

¹Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, ²Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center UTHealth, ³Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, ⁴Women's Health Institute, Cleveland Clinic Foundation, ⁵Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, ⁶Center for Precision Health, School of Biomedical Informatics and School of Public Health, The University of Texas Health Science Center at Houston

Here, we present a protocol for the generation of induced pluripotent Stem Cells (iPSCs) from Li-Fraumeni Syndrome (LFS) patient derived fibroblasts, differentiation of iPSCs via mesenchymal stem cells (MSCs) to osteoblasts, and modeling in vivo tumorigenesis using LFS patient-derived osteoblasts.

Immunology and Infection

Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System

Daniel E. Morales-Mantilla^1,2, Xinyan Huang^3,7, Philip Erice^1,3, Paul Porter⁴, Yun Zhang^1,5, Mary Figueroa⁶, Joya Chandra⁶, Katherine Y. King², Farrah Kheradmand^4,8, Antony Rodríguez^3,8

¹Program in Immunology, Baylor College of Medicine, ²Department of Pediatrics, Section of Infectious Diseases, Baylor College of Medicine, ³Department of Medicine - Immunology Allergy and Rheumatology, Baylor College of Medicine, ⁴Department of Medicine, Pulmonary, Critical Care, Sleep Medicine, Baylor College of Medicine, ⁵Department of Pathology and Immunology, Baylor College of Medicine, ⁶Department of Pediatrics, Research and Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, ⁷Division of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Sun Yat-sen University, ⁸Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey VA Medical Center

This protocol demonstrates the study of the pathophysiologic effects of cigarette smoke (CS) with a whole-body inhalation (WBI) exposure system (WBIS) built in-house. This system can expose animals to CS under controlled repeatable conditions for research of CS-mediated effects on lung emphysema and hematopoiesis.

Medicine

Use of the Scissor-Type Knife During the Peroral Endoscopy Myotomy Procedure for the Treatment of Achalasia

Zhen-Zi Huang^*1, Yuan-Qi Li^*1, Hong-Zhan Tian¹, Yi Cui¹, Min-Hu Chen¹, Xiang-Bin Xing¹

¹Department of Gastroenterology, The First Affiliated Hospital of Sun Yat-sen University

To minimize the technical difficulty and improve the safety of peroral endoscopic myotomy (POEM), we describe a protocol for using a scissor-type knife for the main steps of POEM, including mucosal incision, submucosal tunneling, myotomy, and hemostasis.

Medicine

Vessel-Sparing Microsurgical Longitudinal Intussusception Vasoepididymostomy to Treat Epididymal Obstructive Azoospermia

Ming-kuan Zhou^*1, Jin-tao Zhuang^*1, Wu-yuan Liao², Shi-ying Long³, Xiang-an Tu¹

¹Department of Urology, The East Division of the First Affiliated Hospital of Sun Yat-Sen University, ²Department of Urology, The First Affiliated Hospital of Sun Yat-Sen University, ³Center for Reproductive Medicine, Affiliated Hospital of Guilin Medical University

The present protocol describes a vessel-sparing, longitudinal intussusception vasoepididymostomy using readily available single-needle sutures in China, a safe and effective procedure, which may improve patients' patency and natural pregnancy rates.

Biology

Three-Dimensional Imaging of Aortic Tissues in Atherosclerosis

Ting Sun^1,2,3, Mohammad R. Monjezi², Xu Xu², Changjun Yin^1,2,3,4, Andreas J.R. Habenicht^2,4, Sarajo K. Mohanta^2,4

¹Division of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, ²Institute for Cardiovascular Prevention (IPEK), Ludwig-Maximilians-University, ³Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-sen University, ⁴German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance

Here, we present optimized tissue-clearing protocols to image the murine aorta in three dimensions (3D). We delineate state-of-the-art procedures for immunostaining, optical clearing, and imaging with the intent to define the anatomical proximity of the peripheral nervous system with atherosclerotic plaques and the adventitia in atherosclerosis.

Medicine

Establishment of an Animal Training Model of Duodenoscopy, Choledochoscopy, and Laparoscopy for Cholelithiasis

Baifeng Qian^*1, Junlong Lin^*1, Jianpeng Cai¹, Zhichao Li¹, Yunpeng Hua¹

¹Center of Hepato-Pancreato-Biliary Surgery, The First Affiliated Hospital of Sun Yat-Sen University

Here, we present a protocol for establishing a multi-scopy combined animal training model to enhance the proficiency and comprehension of young doctors in multi-scopy combined technology.