Monitoring Leucine-Rich Repeat Containing 8 Channel (LRRC8/VRAC) Activity using Sensitized-Emission Förster Resonance Energy Transfer (SE-FRET)Malte Klüssendorf 1, Sumaira Pervaiz 2, Tobias Stauber 1,2
1Institute for Molecular Medicine, MSH Medical School Hamburg, 2Institute of Chemistry and Biochemistry, Freie Universität Berlin
Electrophysiology is the gold standard for investigating ion channel activity. However, there are plenty of alternative approaches, including optical methods. Here, we describe a method to monitor the activity of the leucine-rich repeat containing 8 channel (LRRC8)-formed anion channels using an inter-subunit Förster resonance energy transfer (FRET)-based method.
