Microbiota of Attine Ants' Gardens: Visualizing a Microbial Landscape by Scanning Electron MicroscopyMariana Oliveira Barcoto 1, Raphael Affonso Pereira Silva 1, Elliot Watanabe Kitajima 2, Andre Rodrigues 1
1Institute of Biosciences, Rio Claro, Department of General and Applied Biology, São Paulo State University (UNESP), 2Plant Pathology and Nematology Department, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo (USP)
We propose an optimized Scanning Electron Microscopy protocol for visualizing highly heterogeneous and delicate samples containing plant and fungal biomass, together with microbiota and biofilm. This protocol allows describing the spatial dimensions of the microbiota organization.
