Isolation of Chondrocytes and Chondroprogenitors using Fibronectin Adhesion and Migratory AssayElizabeth Vinod 1,2, Ganesh Parasuraman 2, Abel Livingston 3, Solomon Sathishkumar 1, Boopalan Ramasamy 4,5
1Department of Physiology, Christian Medical College, 2Centre for Stem Cell Research (A unit of InStem, Bengaluru), Christian Medical College, 3Department of Orthopedics, Christian Medical College, 4Faculty of Health and Medical Sciences, The University of Adelaide, 5Department of Orthopedics and Trauma, Royal Adelaide Hospital
This protocol details the isolation of chondrocytes, Fibronectin Adhesion Assay-derived Chondroprogenitors (FAA-CPs), and Migratory Chondroprogenitors (MCPs) from human articular cartilage. It covers enzymatic digestion, fibronectin adhesion, and migration-based assays for isolating and characterizing these cells.
