Nantes Université, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Formation of Human Thymus Organoids in Three-Dimensional Fibrin Hydrogels

Manon d'Arco^*1, Nathan Provin^*1, Pierre Maminirina², Olivier Baron², Carole Guillonneau¹, Laurent David^1,3, Matthieu Giraud¹

¹Nantes Université, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ²CHU Nantes, Nantes Université, Department of Pediatric and Congenital Cardiac Surgery, ³Nantes Université, CHU Nantes, INSERM, CNRS, BioCore, SFR Bonamy

Here, we describe a protocol for the formation of human iPSC-derived thymus organoids grown in 3D fibrin hydrogels aiming to support thymic epithelial cell (TEC) maturation and prolonged maintenance, as well as thymopoiesis in vitro.