Formation of Human Thymus Organoids in Three-Dimensional Fibrin HydrogelsManon d'Arco *1, Nathan Provin *1, Pierre Maminirina 2, Olivier Baron 2, Carole Guillonneau 1, Laurent David 1,3, Matthieu Giraud 1
1Nantes Université, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, 2CHU Nantes, Nantes Université, Department of Pediatric and Congenital Cardiac Surgery, 3Nantes Université, CHU Nantes, INSERM, CNRS, BioCore, SFR Bonamy
Here, we describe a protocol for the formation of human iPSC-derived thymus organoids grown in 3D fibrin hydrogels aiming to support thymic epithelial cell (TEC) maturation and prolonged maintenance, as well as thymopoiesis in vitro.