Hyperpolarized 129Xe Lung MRI and Spectroscopy in Mechanically Ventilated MiceMariah L. Costa 1,2,3, Joseph W. Plummer 1,2, Abdullah S. Bdaiwi 1, Brice J. Albert 4, Elizabeth M. Fugate 3, Peter J. Niedbalski 5,6,7, Diana M. Lindquist 3,8, Zackary I. Cleveland 1,2,3,8,9
1Center for Pulmonary Imaging Research, Division of Pulmonary Medicine, Cincinnati Children's Hospital Medical Center, 2Department of Biomedical Engineering, University of Cincinnati, 3Imaging Research Center, Department of Radiology, Cincinnati Children's Hospital Medical Center, 4Analytical Technology Group, Aurorium, 5Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, University of Kansas Medical Center, 6Department of Bioengineering, University of Kansas, 7Hoglund Biomedical Imaging Center, University of Kansas Medical Center, 8Department of Pediatrics, University of Cincinnati, 9Division of Pulmonary Medicine, Cincinnati Children's Hospital Medical Center
Hyperpolarized xenon MRI can quantify regional lung microstructure (air-space dimensions) and physiology (ventilation and gas exchange) in translational research and clinical care. Although challenging, it can provide comparable pulmonary insights in preclinical studies. This protocol describes the infrastructure and procedures needed to perform routine xenon lung MRI in mice.
