Universidad de Santiago de Chile

2 ARTICLES PUBLISHED IN JoVE

Chemistry

Reducing Willow Wood Fuel Emission by Low Temperature Microwave Assisted Hydrothermal Carbonization

Victoria Knappe^*1, Sebastian Paczkowski^*1, Luis Alonso Diaz Robles², Alain Gonzales², Stefan Pelz¹

¹Department of Forest Utilization & Biomass Fuels and Combustion, University of Applied Science Rottenburg, ²Chemical Engineering Department, Faculty of Engineering, Universidad de Santiago de Chile

A protocol for the emission precursor depletion from low quality biomass by low temperature microwave assisted hydrothermal carbonization treatment is presented. This protocol includes the microwave parameters and the analysis of the biocoal product and process water.

Genetics

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Felipe Muñoz-Guzmán^1,2,3, Pablo Quintrel^1,2, José Benavides-Parra^1,2, Catalina Muñoz-Tapia^2,3,4, Luis F. Larrondo^2,4, Francisco A. Cubillos^1,2,5

¹Facultad de Química y Biología, Departamento de Biología, Universidad de Santiago de Chile, ²ANID-Millennium Science Initiative-Millennium Institute for Integrative Biology (iBio), ³Centro Científico y Tecnológico de Excelencia Ciencia & Vida, Fundación Ciencia & Vida, ⁴Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, ⁵ANID-Millennium Nucleus of Patagonian Limit of Life (LiLi)

This protocol employs a bioluminescent reporter, allowing measurements of transcriptional activity in Saccharomyces eubayanus to monitor the glucose-to-maltose transition, enabling real-time analysis of metabolic adaptations and supporting strain optimization for industrial fermentation under diverse conditions.