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University of California at Berkeley

4 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
Meghan Zuck 1,2, Caroline Feng 2, Kevin Hybiske 1
1Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, 2Program in Infectious Diseases, School of Public Health, University of California at Berkeley

A live cell fluorescent protein based method for illuminating cellular vacuoles (inclusions) containing Chlamydia is described. This strategy enables rapid, automated determination of Chlamydia infectivity in samples and can be used to quantitatively investigate inclusion growth dynamics.

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Engineering

Fabrication of Gate-tunable Graphene Devices for Scanning Tunneling Microscopy Studies with Coulomb Impurities
Han Sae Jung 1,2, Hsin-Zon Tsai 1, Dillon Wong 1, Chad Germany 1, Salman Kahn 1, Youngkyou Kim 1,3, Andrew S. Aikawa 1, Dhruv K. Desai 1, Griffin F. Rodgers 1, Aaron J. Bradley 1, Jairo Velasco Jr. 1, Kenji Watanabe 4, Takashi Taniguchi 4, Feng Wang 1,5,6, Alex Zettl 1,5,6, Michael F. Crommie 1,5,6
1Department of Physics, University of California at Berkeley, 2Department of Chemistry, University of California at Berkeley, 3Department of Chemical and Biomolecular Engineering, University of California at Berkeley, 4National Institute for Materials Science (Japan), 5Materials Sciences Division, Lawrence Berkeley National Laboratory, 6Kavli Energy NanoSciences Institute, University of California at Berkeley and Lawrence Berkeley National Laboratory

This paper details the fabrication process of a gate-tunable graphene device, decorated with Coulomb impurities for scanning tunneling microscopy studies. Mapping the spatially dependent electronic structure of graphene in the presence of charged impurities unveils the unique behavior of its relativistic charge carriers in response to a local Coulomb potential.

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Medicine

Real-Time Dynamic Navigation System for the Precise Quad-Zygomatic Implant Placement in a Patient with a Severely Atrophic Maxilla
Yihan Shen *1, Qinggang Dai *1, Baoxin Tao *1, Wei Huang 2, Feng Wang 2, Kengliang Lan 1, Yuanyuan Sun 1, Xiaowan Ling 1, Lijun Yan 1, Yueping Wang 1, Yiqun Wu 1
1The 2nd Dental Center, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, 2Department of Dental Implantology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology

Here, we present a protocol to achieve precise quad-zygomatic implant placement in patients with severely atrophic maxilla using a real-time dynamic navigation system.

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Biology

Measuring In Vivo Adipose Tissue Kinetics in Humans Using the Deuterium (2H)-Labeling Approach
Dereck Kibodeaux 1, Jonathan Savoie 1, Eric Ravussin 1, Mark Fitch 2, Marc K. Hellerstein 2, Ursula White 1
1Pennington Biomedical Research Center, Louisiana State University System, 2University of California at Berkeley

Presented here is a protocol to measure in vivo adipose tissue kinetics in humans using the deuterium (2H)-labeling method.

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