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Australian National University

9 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation
Benjamin J. C. Quah 1, Christopher R. Parish 1
1Department of Immunology, John Curtin School of Medical Research, Australian National University

CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. As such, when a CFSE-labeled cell divides, its progeny have half the amount of fluorescence, which can thereby be used to assess cell division. This article describes the procedures typically used for labeling mouse lymphocytes with CFSE.

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Behavior

Measuring Sensitivity to Viewpoint Change with and without Stereoscopic Cues
Jason Bell 1,2, Edwin Dickinson 2, David R. Badcock 2, Frederick A. A. Kingdom 3
1Research School of Psychology, Australian National University, 2School of Psychology, University of Western Australia, 3McGill Vision Research, Department of Ophthalmology, McGill University

We discuss a novel method forviewpoint-rotation of visual stimuli, and demonstrate using a mirror stereoscopethe three-dimensional percept of rotation-in-depth. The technique can be used to investigate the role of stereoscopic cues in encoding viewpoint-rotated figures.

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Medicine

Slow-release Drug Delivery through Elvax 40W to the Rat Retina: Implications for the Treatment of Chronic Conditions
Lavinia Fiorani *1, Rita Maccarone *1, Nilisha Fernando 2,3, Linda Colecchi 1, Silvia Bisti 1,2, Krisztina Valter 2,3,4
1Biotechnology and Applied Clinical Sciences Department, University of L'Aquila, 2ARC Centre of Excellence in Vision Science, 3John Curtin School of Medical Research, Australian National University, 4ANU Medical School, Australian National University

This paper details how Elvax 40W can be used as a slow-release method for drug delivery to the adult rat retina. The protocol for preparing, loading, and delivering the drug-resin complex to the eye is described.

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Immunology and Infection

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
Benjamin J. C. Quah 1, Danushka K. Wijesundara 1, Charani Ranasinghe 1, Christopher R. Parish 1
1Department of Immunology, John Curtin School of Medical Research, Australian National University

The ability to monitor T cell responses in detail in vivo is important for the development of our understanding of the immune response. Here we describe the use of fluorescent target arrays (FTAs) in an in vivo T cell assay that assesses >250 parameters simultaneously by flow cytometry.

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Immunology and Infection

In Vivo Assessment of Rodent Plasmodium Parasitemia and Merozoite Invasion by Flow Cytometry
Patrick M. Lelliott 1, Brendan J. McMorran 1, Simon J. Foote 1,2, Gaetan Burgio 1
1Australian School of Advanced Medicine, Macquarie University, 2John Curtin School of Medical Research, Australian National University

The malaria parasite invades and replicates within red blood cells. The accurate assessment of merozoite invasion and parasitemia is therefore crucial in assessing the course of malaria infection. Here we describe a flow cytometry based protocol for the measurement of these parameters in a mouse model of malaria.

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Medicine

A Method of Trigonometric Modelling of Seasonal Variation Demonstrated with Multiple Sclerosis Relapse Data
Tim Spelman 1,2, Orla Gray 3, Robyn Lucas 4, Helmut Butzkueven 1,2
1Department of Neurology, Royal Melbourne Hospital, 2Department of Medicine (RMH), The University of Melbourne, 3Department of Neurology, Ulster Hospital, 4National Centre for Epidemiology and Population Health, Australian National University

Combining plot analysis with trigonometric regression is a robust method for exploring complex, cyclical phenomena such as relapse onset timing in multiple sclerosis (MS). This method enabled unbiased characterisation of seasonal trends in relapse onset permitting novel inferences around the influence of seasonal variation, ultraviolet radiation (UVR) and latitude.

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Engineering

Light Enhanced Hydrofluoric Acid Passivation: A Sensitive Technique for Detecting Bulk Silicon Defects
Nicholas E. Grant 1
1Research School of Engineering, Australian National University

A RT liquid surface passivation technique to investigate the recombination activity of bulk silicon defects is described. For the technique to be successful, three critical steps are required: (i) chemical cleaning and etching of silicon, (ii) immersion of silicon in 15% hydrofluoric acid and (iii) illumination for 1 min.

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Neuroscience

Exploring Deep Space - Uncovering the Anatomy of Periventricular Structures to Reveal the Lateral Ventricles of the Human Brain
Alexandru S. Colibaba *1, Aicee Dawn B. Calma *1, Alexandra L. Webb 1, Krisztina Valter 1
1Medical School, College of Medicine, Biology and Environment, Australian National University

This paper demonstrates the effective use of a fiber dissection method to reveal the superficial white matter tracts and periventricular structures of the human brain, in three-dimensional space, to aid student comprehension of ventricular morphology.

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Environment

Techniques for Investigating the Anatomy of the Ant Visual System
Fiorella Ramirez-Esquivel 1, Willi A. Ribi 1, Ajay Narendra 2
1Research School of Biology, Australian National University, 2Department of Biological Sciences, Macquarie University

This article outlines a suite of techniques in light and electron microscopy to study the internal and external eye anatomy of insects. These include several traditional techniques optimized for work on ant eyes, detailed troubleshooting, and suggestions for optimization for different specimens and regions of interest.

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