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Charles R. Drew University of Medicine and Science

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Split-Luciferase Reassembly Assay to Measure Endoplasmic Reticulum-Mitochondria Contacts in Live Cells
Connie Chen *1, Katherine A. Rafael *1, Ginam Cho 1, Youngshin Lim 1,2
1Department of Pathology, Cedars-Sinai Medical Center, 2Department of Biomedical Science Education, College of Medicine, Charles R. Drew University of Medicine and Science

We have established a split-luciferase reassembly assay to monitor the endoplasmic reticulum-mitochondria contacts in live cells. Using this assay, we describe a protocol to quantitatively measure the level of these inter-organelle couplings in HEK293T cells, under the condition of chemical treatment.

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