Washburn University

2 ARTICLES PUBLISHED IN JoVE

Environment

Reliable Method for Assessing Seed Germination, Dormancy, and Mortality under Field Conditions

Brian A. Pace¹, Helen M. Alexander², D. Jason Emry³, Kristin L. Mercer¹

¹Department of Horticulture and Crop Science, Ohio State University, ²Department of Ecology and Evolutionary Biology, University of Kansas, ³Department of Biology, Washburn University

Here we present a protocol for assessing seed survivorship, germination and dormancy under field conditions using buried, labeled seed strips and tetrazolium chloride (TZ) viability testing.

Biology

Transfection of a Molecular Clone of Naegleria gruberi rDNA into N. gruberi Trophozoites

Brian T. Nguyen¹, Nora M. Chapman², John C. Mullican³, Kristen M. Drescher¹

¹Department of Medical Microbiology and Immunology, Creighton University School of Medicine, ²Department of Pathology and Microbiology, University of Nebraska Medical Center, ³Department of Biology, Washburn University

This protocol describes a methodology to transfect Naegleria gruberi trophozoites with a construct that is maintained throughout passaging trophozoites in vitro, as well as through encystment and excystment.