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University of North Texas Health Science Center

6 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor
Ryan W. Bonvillain 1,2, Michelle E. Scarritt 1, Nicholas C. Pashos 1, Jacques P. Mayeux 1, Christopher L. Meshberger 1, Aline M. Betancourt 1,3, Deborah E. Sullivan 1,3, Bruce A. Bunnell 1,2,4
1Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 2Division of Regenerative Medicine, Tulane National Primate Research Center, 3Department of Microbiology and Immunology, Tulane University School of Medicine, 4Department of Pharmacology, Tulane University School of Medicine

Whole-organ decellularization produces natural biological scaffolds that may be used for regenerative medicine. The description of a nonhuman primate model of lung regeneration in which whole lungs are decellularized and then seeded with adult stem cells and endothelial cells in a bioreactor that facilitates vascular circulation and liquid media ventilation is presented.

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Bioengineering

Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation
Jing Zhu 1, Xiaowei Dong 1
1Department of Pharmaceutical Sciences, College of Pharmacy, University of North Texas Health Science Center

Simple homogenization was used to prepare novel, high-density, lipoprotein-mimicking nanoparticles to encapsulate nerve growth factor. Challenges, detailed protocols for nanoparticle preparation, in vitro characterization, and in vivo studies are described in this article.

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Neuroscience

Translaminar Autonomous System Model for the Modulation of Intraocular and Intracranial Pressure in Human Donor Posterior Segments
Tasneem P. Sharma 1, Stacy M. Curry 1, Husain Lohawala 2, Colleen McDowell 3
1North Texas Eye Research Institute, Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, 2Mechanical Engineer Consultant, 3Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin

We describe and detail the use of the translaminar autonomous system. This system utilizes the human posterior segment to independently regulate the pressure inside the segment (intraocular) and surrounding the optic nerve (intracranial) to generate a translaminar pressure gradient that mimics features of glaucomatous optic neuropathy.

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Biochemistry

Visualizing Yeast Organelles with Fluorescent Protein Markers
Chang-Yue Liu 1, Jing Zhu 1, Zhiping Xie 1
1State Key Laboratory of Microbial Metabolism & Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University

Here we describe the use of a set of fluorescent protein-based organelle markers in live-cell imaging of the budding yeast, Saccharomyces cerevisiae.

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Bioengineering

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs
Meagan J. Makarcyzk 1,2, Zhong Alan Li 1,3, Ilhan Yu 1, Haruyo Yagi 1, Xiurui Zhang 1, Lauren Yocum 1, Eileen Li 1, Madalyn R. Fritch 1, Qi Gao 4, Bruce A. Bunnell 5, Stuart B. Goodman 4,6, Rocky S. Tuan 1,8, Peter G. Alexander 1,7, Hang Lin 1,2,7
1Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 2Department of Bioengineering, University of Pittsburgh Swanson School of Engineering, 3Department of Neurobiology, University of Pittsburgh School of Medicine, 4Department of Orthopaedic Surgery, Stanford University, 5Department of Microbiology, Immunology, and Genetics, University of North Texas Health Science Center, 6Department of Bioengineering, Stanford University, 7McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Medicine, 8The Chinese University of Hong Kong

We provide detailed methods for generating four types of tissues from human mesenchymal stem cells, which are used to recapitulate the cartilage, bone, fat pad, and synovium in the human knee joint. These four tissues are integrated into a customized bioreactor and connected through microfluidics, thus generating a knee joint-on-a-chip.

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Biology

Optimizing Mouse Primary Lens Epithelial Cell Culture: A Comprehensive Guide to Trypsinization
Yu Yu *1, Jinmin Zhang *1, Hongli Wu 1,2
1Pharmaceutical Sciences, College of Pharmacy, University of North Texas Health Science Center, 2North Texas Eye Research Institute, University of North Texas Health Science Center

This manuscript outlines a detailed video protocol for culturing primary lens epithelial cells (LECs), aiming to improve reproducibility and aid research in cataracts and posterior capsule opacification (PCO). It offers step-by-step instructions on lens dissection, LECs isolation, and validation, serving as a valuable guide, especially for newcomers in the field.

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