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Donald Danforth Plant Science Center

5 ARTICLES PUBLISHED IN JoVE

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Biology

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays
Ying Deng *1, Guohong Mao *1, William Stutz 1, Oliver Yu 1
1Donald Danforth Plant Science Center

We demonstrate how hairy root composite plants can be used to study plant-rhizobium interactions and nodulation in the difficult-to-transform species Medicago truncatula.

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Environment

Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses
Hui Jiang 1, Hugues Barbier 2, Thomas Brutnell 1
1Donald Danforth Plant Science Center, 2Boyce Thompson Institute

We have developed a methodology for performing crosses in Setaria viridis (S. viridis). The method involves pruning the panicle prior to a hot water treatment to kill viable pollen. Crosses are performed following a well-controlled growth regime and typically result in the recovery of 1 to 7 cross-pollinated seed/s per panicle.

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Biology

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
Kazi T. Islam 1, Dilip M. Shah 1, Kaoutar El-Mounadi 2
1Donald Danforth Plant Science Center, 2Department of Biology, Kutztown University of Pennsylvania

Plant defensins play an important role in plant defense against pathogens. For effective use of these antifungal peptides as antifungal agents, understanding their modes of action (MOA) is critical. Here, a live-cell imaging method is described to study critical aspects of the MOA of these peptides.

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Medicine

Construction and Evaluation of a Murine Calvarial Osteolysis Model by Exposure to CoCrMo Particles in Aseptic Loosening
Hui Jiang *1, Yicun Wang *1, Zhantao Deng *1,2,3, Jiewen Jin 2,3, Jia Meng 1, Shuo Chen 1, Jun Wang 1, Yang Qiu 1, Ting Guo 1, Jianning Zhao 1
1Department of Orthopedics, Jinling Hospital, School of Medicine, Nanjing University, 2Center for Translational Medicine, Nanjing University Medical School, 3Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School

This manuscript describes a murine calvarial osteolysis model by exposure to CoCrMo particles, which constitutes an ideal animal model for assessing the interactions between wear particles and various cells in aseptic loosening.

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Genetics

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Saman Saeidi *1, Michael R. McKain *1,2, Elizabeth A. Kellogg 1
1Donald Danforth Plant Science Center, 2Department of Biological Sciences, The University of Alabama

This article demonstrates a detailed protocol for DNA isolation and high-throughput sequencing library construction from herbarium material including rescue of exceptionally poor-quality DNA.

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